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KMID : 0613820220320120938
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Journal of Life Science
2022 Volume.32 No. 12 p.938 ~ p.946
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MMP-1 and PIP expressions from Ethanol Extract of Hydnocarpus anthelmintica Pierre in Human Fibroblast Cells
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Choi Eun-Young
Jang Young-Ah
Kim Se-Gie
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Abstract
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This study aims to evaluate the effects of antioxidant activities, protein and mRNA expressions of matrix metalloproteinase (MMP) -1 and procollagen type I C-peptide (PIP) in 70% ethanol extract from Hydnocarpus anthelmintica Pierre (HE). DPPH and ABTS+ radicals scavenging assays were measured for antioxidant activities and HE had 73.5% and 74.4% of scavenging activities at 1,000 μg/ml concentration, respectively. And we investigated the inhibition of collagenase by HE, and the result was a 78.8% inhibition effect on concentrations of 1,000 μg/ml. In addition, an MTT assay was performed to confirm the toxicity of the CCD-986sk fibroblasts to the HE, and as a result, the cell viability rate was about 91.7% at a concentration of 50 μg/ml or less, and subsequent cell experi- ments were performed at a concentration of 50 μg/ml or less. We treated the cells with UVB (20 mJ/cm2) for stimulation, treated HE at various concentrations, and performed ELISA tests and RT-PCR experiments. And HE increased the PIP and mRNA in a dose-dependent manner and showed an ex- pression rate of about 64.2% and 83.4%, respectively, at a concentration of 50 μg/ml compared with Cont (50.3% and 45.8%, respectively). And HE suppressed the MMP-1 protein and mRNA in a dose-dependent manner and showed a low expression rate of about 48.7% and 35.9%, respectively, at a concentration of 50 μg/ml. These results can be applied to developing anti-wrinkle materials for functional food and cosmetics with HE.
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KEYWORD
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Hydnocarpus anthelmintica Pierre, matrix metalloproteinase-1, ntioxidant, procollagen type I C-peptide
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